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| Roussel, Erwan; Sauvadet, Anne-laure; Chaduteau, Carine; Fouquet, Yves; Charlou, Jean-luc; Prieur, Daniel; Cambon, Marie-anne. |
| P>The distribution of the archaeal communities in deep subseafloor sediments [0-36 m below the seafloor (mbsf)] from the New Caledonia and Fairway Basins was investigated using DNA- and RNA-derived 16S rRNA clone libraries, functional genes and denaturing gradient gel electrophoresis (DGGE). A new method, Co-Migration DGGE (CM-DGGE), was developed to access selectively the active archaeal diversity. Prokaryotic cell abundances at the open-ocean sites were on average similar to 3.5 times lower than at a site under terrestrial influence. The sediment surface archaeal community (0-1.5 mbsf) was characterized by active Marine Group 1 (MG-1) Archaea that co-occurred with ammonia monooxygenase gene (amoA) sequences affiliated to a group of uncultured... |
| Tipo: Text |
Palavras-chave: Marine subsurface sediments; Sea floor biosphere; Gradient gel electrophoresis; Microbial communities; Molecular diversity; Anaerobic oxidation; Sequence alignment; Phylogenetic trees; Extracellular DNA; Methane hydrate. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6801.pdf |
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| Lazar, Cassandre Sara. |
| A large portion of the greenhouse gas methane on Earth is trapped in marine sediments. However, little methane actually reaches the hydrsphere and the atmosphere, because it is efficiently consumed by anaerobic methane-oxidizing Archaea (ANME). This methane is mainly biogenic in marine sédiments, and is produced by methanogenic Archaea. Methane ascends from deep sources to the seabed, in cold seep sites of continental margins. In order to study microbial community diversity in cold seeps, and to determine geochemical factors that control these microbial communities, we compared four geochemically and geologically distinct sites. In this work, molecular tools (PCR, RT-PCR, DGGE, cloning) as well as genetic markers (16S rRNA, mcrA, dsrB) were employed, to... |
| Tipo: Text |
Palavras-chave: Diversité moléculaire; Archaea; Méthanogène; ANME; McrA; DsrB; DGGE; Volcan de boue; Pockmark; Saumures; Fluides froids; Méthane; Mer de Norvège; Mer Méditerranée; Sédiments.; Molecular diversity; Archaea; Methanogen; ANME; McrA; DsrB; DGGE; Mud volcano; Pockmark; Brines; Cold seep; Methane; Norwegian Sea; Mediterranean Sea; Sediments.. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00031/14250/11534.pdf |
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| Farias, Natanael Dantas; Falchetti, Marcelo; Matos, Gabriel Machado; Schmitt, Paulina; Barreto, Cairé; Argenta, Nicolas; Rolland, Jean-luc; Bachère, Evelyne; Perazzolo, Luciane Maria; Rosa, Rafael Diego. |
| Stylicins are anionic antimicrobial host defense peptides (AAMPs) composed of a proline-rich N-terminal region and a C-terminal portion containing 13 conserved cysteine residues. Here, we have increased our knowledge about these unexplored crustacean AAMPs by the characterization of novel stylicin members in the most cultivated penaeid shrimp, Litopenaeus vannamei. We showed that the L. vannamei stylicin family is composed of two members (Lvan-Stylicin1 and Lvan-Stylicin2) encoded by different loci which vary in gene copy number. Unlike the other three gene-encoded antimicrobial peptide families from penaeid shrimp, the expression of Lvan-Stylicins is not restricted to hemocytes. Indeed, they are also produced by the columnar epithelial cells lining the... |
| Tipo: Text |
Palavras-chave: Crustacean; Penaeid shrimp; Invertebrate immunity; Antimicrobial peptide; Host defense peptide; Molecular diversity. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00466/57816/60102.pdf |
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| Araújo,Roberta A.C.; Gomes,Fátima C.O.; Moreira,Elizabeth S.A.; Cisalpino,Patrícia S.; Rosa,Carlos A.. |
| An ecological study on Saccharomyces cerevisiae populations in spontaneous fermentation has been conducted in three vats of a cachaça distillery in Minas Gerais, Brazil. Ninety-seven yeast isolates were collected at the beginning, the middle and at the end of the production period, and were identified by standard methods. Differentiation between the indigenous S. cerevisiae strains isolated was performed by mitochondrial DNA (mtDNA) restriction analysis, RAPD-PCR, and PCR fingerprint using an intron splice primer. Analysis of the mtDNA restriction profiles revealed 12 different patterns, 11 corresponding to indigenous yeasts (I to XI) and one (XII) to a commercial strain of the bakery yeast. Pattern II (53.6% of the population) and pattern IV strains were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Saccharomyces cerevisiae; Cachaça; Fermentation; Molecular diversity. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200006 |
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| Moreira,Daniel A.; Buckup,Paulo A.; Britto,Marcelo R.; Magalhães,Maithê G. P.; Andrade,Paula C. C. de; Furtado,Carolina; Parente,Thiago E.. |
| ABSTRACT The complete mitogenome of Corydoras nattereri , a species of mailed catfishes from southeastern Brazil, was reconstructed using next-generation sequencing techniques. The mitogenome was assembled using mitochondrial transcripts from the liver transcriptomes of three individuals, and produced a circular DNA sequence of 16,557 nucleotides encoding 22 tRNA genes, two rRNA genes, 13 protein-coding genes and two noncoding control regions (D-loop, OrigL). Phylogeographic analysis of closely related sequences of Cytochrome Oxydase C subunit I (COI) demonstrates high diversity among morphologically similar populations of C. nattereri . Corydoras nattereri is nested within a complex of populations currently assigned to C. paleatus and C. ehrhardti .... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Barcode; DNA; Mitogenome; Molecular diversity; MtRNA.. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000100218 |
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